α-Synuclein binding assay to isolated synaptic vesicles

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Description

The binding of α-synuclein (α-syn) to synaptic vesicles was assessed by pull-down using C-terminally His-tagged α-syn and Co Dynabeads (10103D, Invitrogen). Synaptic vesicles (SVs) were isolated from mouse brain (LP2 fraction), incubated with recombinant α-syn, and the bound fractions were analyzed by immunoblotting.

Identifier (DOI)

10.17504/protocols.io.36wgqp8mxvk5/v1

Discovery Team

Team Edwards

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Akio Mori

External Collaborator
Robert Edwards, MD

Lead PI (Core Leadership): Team Edwards,

University of California, San Francisco

α-Synuclein binding assay to isolated synaptic vesicles

Output Details

Meet the Authors

Akio Mori

Robert Edwards, MD

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