3′ mRNA-sequencing

View Published Protocol

Output Details

Description
Protocol for 3' mRNA sequencing of mouse gastrointestinal tract tissues
Identifier (DOI)
10.17504/protocols.io.bzqbp5sn
Tags
  • Activity Assay
  • Gut
  • Mouse
  • RNA-seq
Team
Team Gradinaru and Team Sulzer
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions Neuro-Immune Interactions

Related Research

Peripheral neuronal activation shapes the microbiome and alters gut physiology

The authors specifically activate ChAT- or TH-expressing gut-associated neurons in mice and perform multi-omics, finding that subsets of peripherally-activated neurons differentially regulate the gut microbiome and host GI physiology.

View Article

Lysosomal GCase (glucocerebrosidase) activity assay

Method to measure lysosomal GBA1 enzyme activity using 4-MUG substrate at low pH to minimize non-lysosomal GCase interference. GBA1 activity higher in purified lysosomes than whole cell extract, confirmed by CBE inhibitor abolishing 4-MUG hydrolysis.

View Protocol

Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology

This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including voltage clamp and current clamp recordings as well as optogenetic stimulation in the Ding lab.

View Protocol
View More Outputs