Chemogenetic modulation of catecholaminergic neurons
Output Details
Description
This protocol outlines a method for modulating noradrenergic and dopaminergic neuronal activity in the LC and SNc-VTA, respectively, using Cre-dependent hM3D and hM4D expression. It details stereotactic injection techniques for these chemogenetic tools and the preparation of the J60 agonist for intraperitoneal delivery. Developed for longitudinal studies spanning up to four weeks, the protocol aims to assess neuromelanin's activity-dependent accumulation through daily agonist administration.
Identifier (DOI)
10.17504/protocols.io.rm7vzxdz5gx1/v1
