Cloning, Protein Expression, and Purification of 20S CPs and Assembly Intermediates

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Description

This protocol details methods for cloning, expression, and purification of 20S CPs and assembly intermediates for biochemical and structural analysis.

Identifier (DOI)

10.17504/protocols.io.n92ldmd59l5b/v1

Tags

Cloning
Protein purification
Structural biology

Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Frank Adolf, PhD

Key Personnel: Team Harper,

MPI

Cloning, Protein Expression, and Purification of 20S CPs and Assembly Intermediates

Output Details

Meet the Authors

Frank Adolf, PhD

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