Our Year in Review: Insights, outcomes, and highlights that shaped 2025. Read the full report!
Aligning Science Across Parkinson's Logo Text

Confocal imaging of transcriptional crosstalk between AAV genomes carrying different enhancers in the mouse brain

By Gerard Coughlin, BSc, Mate Borsos, Nathan Appling, Bre'Anna Barcelona, Acacia Mayfield, PhD, Elisha Mackey, Rana Eser, Cameron Jackson, Xinhong Chen, Spripriya Ravindra Kumar, Viviana Gradinaru, PhD
View Published Dataset

Output Details

Description
This data collection contains images of brains of mice transduced with viral vectors carrying different regulatory elements and reporters to study transcriptional crosstalk between AAV genomes. The data is described in the publication "Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo."
Identifier (DOI)
10.35077/g.1163
Tags
  • AAV (Adenoassociated Virus)
  • Brain
  • Microscopy - optical
  • Mouse
  • Transcriptional regulation
Team
Team Gradinaru
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Gerard Coughlin, BSc

    Key Personnel: Team Gradinaru, 

    California Institute of Technology

  • User avatar fallback logo

    Mate Borsos

    External Collaborator

  • User avatar fallback logo

    Nathan Appling

    External Collaborator

  • User avatar fallback logo

    Bre'Anna Barcelona

    External Collaborator

  • User avatar fallback logo

    Acacia Mayfield, PhD

    Key Personnel: Team Gradinaru, 

    California Institute of Technology

  • User avatar fallback logo

    Elisha Mackey

    External Collaborator

  • User avatar fallback logo

    Rana Eser

    External Collaborator

  • User avatar fallback logo

    Cameron Jackson

    External Collaborator

  • User avatar fallback logo

    Xinhong Chen

    External Collaborator

  • User avatar fallback logo

    Spripriya Ravindra Kumar

    External Collaborator

  • Viviana Gradinaru, PhD

    Lead PI (Core Leadership): Team Gradinaru, 

    California Institute of Technology

Related Research

pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

View Lab Material

Ef1a-DIO-PSAML-141F,Y115F-GlyR-2A-FusionRed plasmid

Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

View Lab Material

pAAV-Con/Fon-N2cG

This plasmid was generated by Columbia University Zuckerman Institute Molecular Tools Core Facility (RRID:SCR_026201) for our study and injected to label presynaptic inputs to SNr neurons.

View Lab Material
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.