Cryo-electron tomography of thinned synapses
Output Details
Description
Here we provide a protocol for cryo-electron tomography (cryo-ET) of thinned synapses within rat primary neuron cultures. This workflow relies on cryo-focused-ion-beam (FIB) milling to enable unrestricted access to synapses within neuronal cultures and to achieve samples sufficiently thin (~150 nm) lamellae for high resolution cryo-ET imaging. Synapse targeting with and without cryo-fluorescence light microscopy (cryo-FLM) correlation for FIB milling and cryo-ET is introduced.
Identifier (DOI)
10.17504/protocols.io.n92ldm188l5b/v1
