Aggregates of α-Synuclein are linked to Parkinson’s, multiple system atrophy, and Lewy body dementia. This protocol outlines near-atomic resolution structures of these amyloid fibrils using cryo-EM single particle analysis.
3.0 A resolution cryo-EM map obtained from recombinant a-synuclein fibrils carrying the G14R mutation recently discovered in a patient of a complex neurological disease.