Dephosphorylation of phosphorylated Rab GTPases by PPM phosphatases

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Output Details

Description
This protocol describes our method to measure phosphoRab GTPase dephosphorylation using purified phosphoRab GTPases and PPM family phosphatases. We include our protocol for generation of phosphatase substrates by phosphorylation of Rab GTPases by MST3 kinase.
Identifier (DOI)
10.17504/protocols.io.5jyl8d4j7g2w/v1
Tags
  • Activity Assay
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

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