Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector

By Justin Savage, BSc
View Published Protocol

Output Details

Description
This protocol describes designing sgRNAs for genes of interest using the GEARBOCS vector backbone. This method allows researchers to utilize the GEARBOCS system for astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays
Identifier (DOI)
10.17504/protocols.io.5jyl8271rl2w/v1
Tags
  • Astrocytes
  • gRNA oligonucleotides
  • Other
  • sgRNA
  • Viral vectors
Team
Team Calakos
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Justin Savage, BSc

    Key Personnel: Team Calakos, 

    Duke University

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