Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence

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Description

This protocol describes the evaluation of cellular processing of GPNMB mutants by Western Blotting and Immunofluorescent imaging in a HEK293 overexpression model.

Identifier (DOI)

10.17504/protocols.io.4r3l22yz4l1y/v1

Tags

Cell culture
GPNMB
Immunofluorescence
Other
Transfection
Western Blot

Team

Team Hardy

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Erin Bogacki, MSc

Key Personnel: Team Hardy,

Royal Veterinary College
Patrick Lewis, PhD

Collaborating PI: Team Hardy,

Royal Veterinary College
Susanne Herbst, PhD

Key Personnel: Team Hardy,

Royal Veterinary College

Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence

Output Details

Meet the Authors

Erin Bogacki, MSc

Patrick Lewis, PhD

Susanne Herbst, PhD

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