Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence

By Erin Bogacki, MSc, Patrick Lewis, PhD and Susanne Herbst, PhD
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Output Details

Description
This protocol describes the evaluation of cellular processing of GPNMB mutants by Western Blotting and Immunofluorescent imaging in a HEK293 overexpression model.
Identifier (DOI)
10.17504/protocols.io.4r3l22yz4l1y/v1
Tags
  • Cell culture
  • GPNMB
  • Immunofluorescence
  • Other
  • Transfection
  • Western Blot
Team
Team Hardy
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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    Erin Bogacki, MSc

    Key Personnel: Team Hardy, 

    Royal Veterinary College

  • Patrick Lewis, PhD

    Collaborating PI: Team Hardy, 

    Royal Veterinary College

  • Susanne Herbst, PhD

    Key Personnel: Team Hardy, 

    Royal Veterinary College

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Dataset associated with DOI: 10.17617/3.51VXU0

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