Figure 7. Pacer expression promotes formation of WIPI2 puncta upon depolarization [FINAL]

Figure 7. Pacer expression promotes formation of WIPI2 puncta upon depolarization (A) Pacer KO HeLa cells expressing Parkin and either wildtype Pacer, mutant PacerK534N, R623T , or empty vector, were depolarized for 1 H using 10 μM Oligomycin A and 5 μM Antimycin A following transient transfection with HaloTag-WIPI2B. (B) For each independent trial, ~20 cells were imaged and the total above-cytosol background, or punctate, WIPI2 signal was measured. Three trials were performed, and the trials performed in parallel are indicated by colors. Trial averages are indicated by bolded dots, and horizontal bars indicate averages across all three trials. P-value is the result of a one-tailed, paired T-test between the trial averages in each category. (C) Quantification of the number of puncta positive for both Pacer and WIPI2 on a per cell basis. Replicates are indicated as in panel B, and P-value is also the result of a one-tailed, paired T-test.