ASAP announces new teams joining the Collaborative Research Network! Learn More
Aligning Science Across Parkinson's Logo Text

Formation and isolation of Clu phospholipid particles

By Patricia Yuste Checa, Andreas Bracher, Franz-Ulrich Hartl, MD
View Published Protocol

Output Details

Description
This protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).
Identifier (DOI)
10.17504/protocols.io.bp2l6x59zlqe/v1
Tags
  • CLU (Clusterin)
  • Isolation
Discovery Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • User avatar fallback logo

    Patricia Yuste Checa

    External Collaborator

  • User avatar fallback logo

    Andreas Bracher

    External Collaborator

  • Franz-Ulrich Hartl, MD

    Co-PI (Core Leadership): Team Harper, 

    Max Planck Institute of Biochemistry

Related Research

Local genetic correlations exist among neurodegenerative and neuropsychiatric diseases

Genetic correlation between neurodegenerative and neuropsychiatric diseases was explored using local rg analysis. Unique relationships were found, suggesting shared genetic mechanisms and potential therapeutic targets in complex diseases.

View Article

The chaperone Clusterin in neurodegeneration−friend or foe?

The authors review the diverse functions of Clusterin in the pathogenesis of neurodegenerative diseases, focusing on evidence that Clusterin may act either as a suppressor or enhancer of pathology.

View Article

HEK293E_Clu TL1

Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

View Lab Material
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.