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Formation and isolation of Clu phospholipid particles

By Patricia Yuste Checa, PhD, Andreas Bracher and Franz-Ulrich Hartl, MD
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Output Details

Description
This protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).
Identifier (DOI)
10.17504/protocols.io.bp2l6x59zlqe/v1
Tags
  • CLU (Clusterin)
  • Isolation
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Patricia Yuste Checa, PhD

    Key Personnel: Team Harper, 

    Max Planck Institute of Biochemistry

  • User avatar fallback logo

    Andreas Bracher

    External Collaborator

  • Franz-Ulrich Hartl, MD

    Co-PI (Core Leadership): Team Harper, 

    Max Planck Institute of Biochemistry

Related Research

Local genetic correlations exist among neurodegenerative and neuropsychiatric diseases

Genetic correlation between neurodegenerative and neuropsychiatric diseases was explored using local rg analysis. Unique relationships were found, suggesting shared genetic mechanisms and potential therapeutic targets in complex diseases.

View Article

The chaperone Clusterin in neurodegeneration−friend or foe?

The authors review the diverse functions of Clusterin in the pathogenesis of neurodegenerative diseases, focusing on evidence that Clusterin may act either as a suppressor or enhancer of pathology.

View Article

HEK293E_Clu TL1

Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

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Aligning Science Across Parkinson's
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