GCase assay – Resorufin β-D-glucopyranoside
Output Details
Description
We present a method for assessing the enzyme activity of lysosomal glucocerebrosidase (GBA1, GCase) in proteinlysates by monitoring the hydrolysis of the fluorescent substrate Resorufin β-D-glucopyranoside. The assay isconducted at low pH, where non-lysosomal glucocerebrosidase activity is expected to be minimal. This approachis supported by the complete loss of the fluorescent signal in GBA1 knockout (KO) cells. The assay is compatiblewith cell and tissue lysates, as well as organellar immunoprecipitations. Given the variable expression of GBA1across different tissues and cell types, the amount of protein used in the assay can be adjusted accordingly.
Identifier (DOI)
10.17504/protocols.io.n2bvj9kpplk5/v1
