GFP-Clu-tails purification from Escherichia coli cells

By Patricia Yuste Checa, PhD and Franz-Ulrich Hartl, MD
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Output Details

Description
This protocol details how to purify the fusion protein GFP-Clu-tails from Escherichia coli.
Tags
  • CLU (Clusterin)
  • Protein purification
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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HEK293E_Clu WT

Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

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