This protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for…
Cholinergic neurons (CNs) in the pedunculopontine nucleus (PPN) are lost in the course of Parkinson’s disease. PPN CNs have a distinctive physiological phenotype that shares only some of the features of other selectively vulnerable neurons in PD.
