This protocol details the preparation of alpha synuclein preformed fibrils for in vitro treatment to cells for the analysis of protein uptake and processing or clearance.
This pilot establishes brain organoids from iPSC-derived vmDA neurons, optimizing growth conditions and exploring tri-culture integration with microglia to model neurodevelopment and cell-type interactions.
Proteomic analysis of iNeurons and cells lacking ER-phagy receptors FAM134C, FAM134A, FAM134B, TEX264, and CCPG1 showed distinct protein profiles, highlighting their roles in ER-phagy pathways.
