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N_terminal protein labeling

By Patricia Yuste Checa, PhD, Franz-Ulrich Hartl, MD
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Output Details

Description
This protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.
Identifier (DOI)
10.17504/protocols.io.rm7vzxpbrgx1/v1
Tags
  • CLU (Clusterin)
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

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Genetic correlation between neurodegenerative and neuropsychiatric diseases was explored using local rg analysis. Unique relationships were found, suggesting shared genetic mechanisms and potential therapeutic targets in complex diseases.

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The chaperone Clusterin in neurodegeneration−friend or foe?

The authors review the diverse functions of Clusterin in the pathogenesis of neurodegenerative diseases, focusing on evidence that Clusterin may act either as a suppressor or enhancer of pathology.

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HEK293E_Clu TL1

Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

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Aligning Science Across Parkinson's
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