Preparing plasmids for nucleofection of hPSCs

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Description

This protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.

Identifier (DOI)

10.17504/protocols.io.b4pdqvi6

Tags

DNA
Human
In Vitro
Nucleofection
Plasmids

Discovery Team

Team Rio

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Oriol Busquets Figueras

External Collaborator
Hanqin Li

External Collaborator
Steven Poser

External Collaborator
Frank Soldner, MD

Co-PI (Core Leadership): Team Rio,

Albert Einstein College of Medicine
Dirk Hockemeyer, PhD

Co-PI (Core Leadership): Team Rio,

University of California, Berkeley

Preparing plasmids for nucleofection of hPSCs

Output Details

Meet the Authors

Oriol Busquets Figueras

Hanqin Li

Steven Poser

Frank Soldner, MD

Dirk Hockemeyer, PhD

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