Preparing plasmids for nucleofection of hPSCs

Output Details

This protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.

Tags
  • DNA
  • Human
  • In Vitro
  • Nucleofection
  • Plasmids

Meet the Authors

  • Oriol Busquets Figueras, PhD

    Key Personnel: Team Rio

    Albert Einstein College of Medicine

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    Hanqin Li, PhD

    Key Personnel: Team Rio

    University of California, Berkeley

  • Steven Poser, PhD

    Key Personnel: Team Rio

    University of Washington

  • Frank Soldner, MD

    Co-PI (Core Leadership): Team Rio

    Albert Einstein College of Medicine

  • Dirk Hockemeyer, PhD

    Co-PI (Core Leadership): Team Rio

    University of California, Berkeley