Proteomics: On-bead/in-solution digestion and phosphopeptide enrichment

View Published Protocol

Output Details

Description
Procedure for On-bead/in-solution digestion and phosphopeptide enrichment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Identifier (DOI)
10.17504/protocols.io.rm7vzx195gx1/v1
Tags
  • Phosphorylation
  • Proteins
  • Proteomics
Team
Team Gradinaru
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Related Research

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function

Phosphorylation of α-synuclein at the serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states, only a fraction of α-syn is phosphorylated at this site, and most…

View Article

Western blotting for LRRK2 signalling in macrophages

This protocol describes the immunoblotting for components of the LRRK2 signalling pathway (LRRK2, LRRK2 pS935 and phospho-Rabs) using Invitrogen NuPage SDS-PAGE reagents and the BioRad Turbo Blot transfer system.

View Protocol

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function

Phosphorylation of α-synuclein at the serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states, only a fraction of α-syn is phosphorylated at this site, and most…

View Article
View More Outputs