Proximity ligation assay

By Isabel Lam, PhD, Alain Ndayisaba, MD and Vikram Khurana, MD
View Published Protocol

Output Details

Description
Proximity ligation assay (PLA) in iPSC-derived cortical neurons. Permeabilization with Saponin detergent is used to preserve lipid-rich inclusions.
Identifier (DOI)
10.17504/protocols.io.kqdg32n57v25/v1
Team
Team Studer
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions

The authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.

View Article

Plasmid construction – Chen et al. 2025 NSMB

Standard cloning procedure.

View Protocol

Image Processing and 3D Reconstruction – Chen et al. 2025 NSMB

Image processing and 3D reconstruction applied in Chen et al. 2025 NSMB

View Protocol
View More Outputs