Rapid Isolation of Lysosomes (LysoIP) from mouse brain tissue

This protocol outlines a method for the rapid isolation of lysosomes from fresh mouse brain tissue. Lysosomes serve as metabolic hubs for the degradation and recycling of biomolecules. Despite their critical cellular functions, quantitatively assessing lysosomal protein profiles has been challenging. To address this, we developed a rapid harvesting and purification method using immunoprecipitation (LysoIP). This protocol provides detailed instructions for preparing LysoIP from mouse brain tissue for proteomic analysis.