SureSelect XT HS2 DNA to prepare libraries for single-cell Whole Genome Sequencing (scWGS) after single-cell Whole Genome Amplification (scWGA)

Description

We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as input material samples after single-cell Whole Genome Amplification (scWGA), instead of genomic DNA samples, for library preparation for Illumina single-cell Whole Genome Sequencing (scWGS). This protocol can be employed either manually (Section 2: Option A) or in combination with automation using the Bravo Automated Liquid Handling Platform (Section 2: Option B).

Identifier (DOI)

10.17504/protocols.io.x54v9p3qzg3e/v1

Tags

DNA WGS
Whole-genome amplification (WGA)

Team

Team Voet

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Ester Kalef-Ezra, PhD

Key Personnel: Team Voet,

University College London
Ben Harvey
Katherine Roper
Christos Proukakis, PhD

Co-PI (Core Leadership): Team Voet,

University College London

SureSelect XT HS2 DNA to prepare libraries for single-cell Whole Genome Sequencing (scWGS) after single-cell Whole Genome Amplification (scWGA)

Output Details

Meet the Authors

Ester Kalef-Ezra, PhD

Ben Harvey

Katherine Roper

Christos Proukakis, PhD

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