Surface protein biotinylation

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Description

This protocol describes surface protein labeling with biotin using EZ-Link Sulfo-NHSLC-Biotin. This chemical reacts with primary amines such as lysine but does not permeate cell membranes because of the charge. Thus, it only biotinylates surface proteins.

Identifier (DOI)

10.17504/protocols.io.b2c8qazw

Team

Team De Camilli

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Daehun Park, PhD

Key Personnel: Team De Camilli,

Yale University
Pietro De Camilli, PhD

Lead PI (Core Leadership): Team De Camilli,

Yale University

Surface protein biotinylation

Output Details

Meet the Authors

Daehun Park, PhD

Pietro De Camilli, PhD

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