TAB-PAINT imaging of alpha-synuclein fibrils using Nile Red

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Description

This is a protocol for the preparation of a sample of alpha-synuclein fibrils on a PLL-coated cover glass for TAB-PAINT imaging with Nile Red. This protocol was used to generate the data shown in Figure 3 of the following publication: Bruggeman et al., POLCAM: Instant molecular orientation microscopy for the life sciences. bioRxiv 2023.02.07.527479 (Feb 2023), doi: https://doi.org/10.1101/2023.02.07.527479

Identifier (DOI)

10.17504/protocols.io.n2bvj31mwlk5/v1

Team

Team Wood

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Ezra Bruggeman, MSc

Key Personnel: Team Wood,

University of Cambridge

TAB-PAINT imaging of alpha-synuclein fibrils using Nile Red

Output Details

Meet the Authors

Ezra Bruggeman, MSc

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