Optimized protocol for generating authentic ventral midbrain dopaminergic neurons from human iPSCs enhances cell therapy and modeling, improves consistency across lines, and supports integration with organoid and assembloid systems.
This pilot establishes brain organoids from iPSC-derived vmDA neurons, optimizing growth conditions and exploring tri-culture integration with microglia to model neurodevelopment and cell-type interactions.
Protocol collection for the study "Genome-wide CRISPR screen reveals neddylation's role in neuronal aging and AD neurodegeneration" by Saurat et al. in Cell Stem Cell 2024. Contains 20 protocols. Full manuscript: DOI: 10.1016/j.stem.2024.06.001.