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TX2P – Transcript to protein

By Emil Gustavsson, PhD, David Murphy, MSc
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Output Details

Description
TX2P allows for automated integration of mass spectometry data in long-read RNA-sequencing workflow. It does so by predicting open reading frames for each transcripts within an input GTF/GFF file, translates it into peptide sequences and then searches for those predicted proteins in mass spec datasets using MetaMorpheus.
Tags
  • Long-read sequencing
  • Mass spectrometry
  • Workflow
Team
Team Wood
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Emil Gustavsson, PhD

    Key Personnel: Team Wood,  Team Hardy, 

    University College London

  • User avatar fallback logo

    David Murphy, MSc

    Key Personnel: Team Wood, 

    University College London

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Long-read RNA seq analysis using Talon

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The annotation of GBA1 has been concealed by its protein-coding pseudogene GBAP1

The authors identify novel transcripts from both GBA1 and GBAP1, including protein-coding transcripts that are translated in vitro and detected in proteomic data, but that lack GCase activity.

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Aligning Science Across Parkinson's
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