Behavior Hardware Setup Protocol
By onProtocol outlines steps for replicating conditioning/extinction reward learning assay.
In vivo Ephys: Analysis Protocol
By onProtocol outlines analysis steps for in vivo electrophysiology data after spike sorting.
In vivo Ephys: Spike Sorting Protocol
By onProtocol outlines spike sorting steps for electrode recording data.
Histology: Analysis Protocol
By onProtocol analyzes histology images using Matlab for defining ROIs and ilastik for pixel classification
Behavior: Analysis Protocol
By onThe protocol outlines how to visualize and analyze reward learning behavior data for research purposes.
Untargeted lipidomics of Tagless Lyso-IP
By onThe authors have developed the Tagless Lyso-IP method to rapidly prepare lysosome-enriched samples from human peripheral blood.
Untargeted metabolomics of Tagless Lyso-IP
By onLysosomal biology is increasingly implicated in neurodegenerative diseases and health. It has traditionally been difficult to profile the metabolomic homeostasis of the lysosome in disease states. To overcome this challenge, we have developed the Tagless Lyso-IP method to rapidly prepare lysosome enriched samples from human peripheral blood. This protocol details the processing and untargeted analysis of polar metabolites derived using the Tagless Lyso-IP method.
Fixation and Immunostaining protocol
By onFixation and immunostaining protocol for tissue and cell culture.
Light microscopy-based neuron tracing and reconstruction
By onProtocol outlines neuron reconstruction as delineating neuron components using light microscopy to create geometric models for quantitative morphology analysis.
Immunohistochemistry data processing
By onThis protocol has been used for high throughput quantification of immunohistochemistry data of mouse brain sections.
Glucosylceramide and glucosylsphingosine analysis V.2
By onThis protocol was used to analysis glucosylceramide and glucosylsphingosine levels in mouse brains and liver. It was also described in a previous publication (Mol Cell Neurosci. 2020 Jan:102:103451, doi: 10.1016).
Quantitative targeted metabolomics for ASO mouse model using Biocrates Q500 platform
By onProtocol for using the biocrates MxP Quant 500 kit for targeted metabolomics of mouse tissues
A CellProfiler computational pipeline to quantify the density of mouse striatal dopaminergic processes
By onHere, we present a CellProfiler (PMID: 34507520) software pipeline to quantify the density and intensity of dopaminergic processes in the mouse striatum. The dopaminergic processes in the striatum are stained using an anti-tyrosine hydroxylase antibody and the same sections are also stained using an antibody that recognizes total neuronal NeuN (neuronal nuclear antigen biomarker) for staining normalization. For the examples shown herein, images were acquired using a Zeiss LSM 900 laser scanning confocal microscope with a 63X 1.4 oil immersion objective.
Cell counting
By onProtocol for counting cells labeled with immunofluorescent markers in mouse brain sections.
