Immunohistochemistry data processing
By onThis protocol has been used for high throughput quantification of immunohistochemistry data of mouse brain sections.
Glucosylceramide and glucosylsphingosine analysis V.2
By onThis protocol was used to analysis glucosylceramide and glucosylsphingosine levels in mouse brains and liver. It was also described in a previous publication (Mol Cell Neurosci. 2020 Jan:102:103451, doi: 10.1016).
Quantitative targeted metabolomics for ASO mouse model using Biocrates Q500 platform
By onProtocol for using the biocrates MxP Quant 500 kit for targeted metabolomics of mouse tissues
A CellProfiler computational pipeline to quantify the density of mouse striatal dopaminergic processes
By onHere, we present a CellProfiler (PMID: 34507520) software pipeline to quantify the density and intensity of dopaminergic processes in the mouse striatum. The dopaminergic processes in the striatum are stained using an anti-tyrosine hydroxylase antibody and the same sections are also stained using an antibody that recognizes total neuronal NeuN (neuronal nuclear antigen biomarker) for staining normalization. For the examples shown herein, images were acquired using a Zeiss LSM 900 laser scanning confocal microscope with a 63X 1.4 oil immersion objective.
Cell counting
By onProtocol for counting cells labeled with immunofluorescent markers in mouse brain sections.
MSD V-PLEX Proinflammatory panel
By onThe V-PLEX panel offers analytically validated multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot.
Electrophysiology with iPSC-derived neurons
By onThis protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.
Protein structure prediction with AlphaFold-Multimer
By onThis protocol describes the protein structure prediction with AlphaFold-Multimer.
IHC and image analysis related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onIHC and image analysis related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Statistical analysis protocol for “”LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onStatistical analysis protocol for ""LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Model Building of PI3KC3-C1/RAB1A
By onDetails model building of the PI3KC3-C1/RAB!A complex using chimeraX1.5-1.6 and ISOLDE. Refinement was done using Phenix real space refinement.
Database-driven protein solubilization
By onThis is a protocol for using the extraction database found at www.polymerscreen.yale.edu for optimal extraction of membrane proteins into native nanodiscs.
