Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
By onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
Analyses of mitochondrial function in SNpc with the Seahorse XFe96
By onProtocol for analyses of mitochondrial function in SNpc with the Seahorse XFe96
Western Blot – Ford Lab
By onThis protocol describes the solutions and steps for performing western blots.
Pipeline for Image Processing and Quantification of Striatal Dopamine Innervation using Fiji (IF/IHC)
By onThis protocol outlines the image preprocessing steps required prior to analysis with ImageJ tools as well as the ImageJ pipeline to quantify striatal innervation.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
By onCellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
Ex Vivo Electrophysiology V2 – Alternate version of Nelson lab protocol
By onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
3D spatial alignment of multiple similar objects extracted from FIB-SEM data using Fijiyama
By onProcedure for the use of Fijiyama ("Yet Another Matching and Alignment tool for Fiji") to spatially align a series of similar cellular structures detected from 3D FIB-SEM data.
Preparation of 3D distance probes for the quantification of variability in distance measurements between conditions
By onSimple procedure for preparing random 3D probe points for the analysis of image segmentation variability
3D distance measurements & rendering with AIVE results
By onProcedure for conducting 3D distance measurements and visualizations (with Paraview and Blender Respectively)
Processing 3D FIB-SEM data via AIVE
By onA complete procedure for processing 3D FIB-SEM data via AIVE (AI-directed Voxel Extraction); from raw images to final result.
Isolation of brain eGFP-cholinergic interneurons by dissociation and flow cytometry
By onProtocol isolates GFP-expressing cholinergic interneurons from mouse dorsal striatum tissue using a method by Lynette Foo (2013).
Immunofluorescence and confocal imaging in brain sections and image analysis
By onImmunofluorescence and confocal imaging in brain sections and image analysis for Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Lipidomic analysis in HeLa cells
By onA protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in HeLa cells for HexCer levels
By onA protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
LC-MS/MS Analysis
By onLC-MS/MS Analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Multicolor fluorescence in situ hybridization and analysis
By onMulticolor fluorescence in situ hybridization and analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Striatal synaptosome preparation for imaging
By onStriatal synaptosome imaging and analysis protocols in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
SIM imaging and analysis for active zone release sites in vulnerable dopamine axons
By onStereotactic injections and viruses described and SIM imaging and analysis
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
By onImmunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
