3D distance measurements & rendering with AIVE results
By onProcedure for conducting 3D distance measurements and visualizations (with Paraview and Blender Respectively)
Processing 3D FIB-SEM data via AIVE
By onA complete procedure for processing 3D FIB-SEM data via AIVE (AI-directed Voxel Extraction); from raw images to final result.
Isolation of brain eGFP-cholinergic interneurons by dissociation and flow cytometry
By onProtocol isolates GFP-expressing cholinergic interneurons from mouse dorsal striatum tissue using a method by Lynette Foo (2013).
Immunofluorescence and confocal imaging in brain sections and image analysis
By onImmunofluorescence and confocal imaging in brain sections and image analysis for Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Lipidomic analysis in HeLa cells
By onA protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in HeLa cells for HexCer levels
By onA protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
LC-MS/MS Analysis
By onLC-MS/MS Analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Multicolor fluorescence in situ hybridization and analysis
By onMulticolor fluorescence in situ hybridization and analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Striatal synaptosome preparation for imaging
By onStriatal synaptosome imaging and analysis protocols in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
SIM imaging and analysis for active zone release sites in vulnerable dopamine axons
By onStereotactic injections and viruses described and SIM imaging and analysis
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
By onImmunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
Quantification of Axonal Projections from Whole-Brain Slide Images
By onQuantification of Axonal Projections from Whole-Brain Slide Images provides a standardized method for processing and analyzing immunofluorescence images of mouse brain sections to quantify axonal projections.
A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons
By onBrain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.
Whole-cell patch-clamp recordings
By onThis protocol describes the preparation of hippocampal slices from mice for whole-cell patch-clamp recordings and technique for whole-cell recordings from CA1 pyramidal neurons.
Measurement of Oxygen Consumption Rate (OCR) in iPSC-Derived Neurons and iPSCs Using Seahorse XFe96 Analyzer
By onProtocol measures OCR in iPSC-derived neurons & undifferentiated iPSCs using Seahorse XFe96 Analyzer.
Protein analyses and immunoblotting
By onProtocol for Western blotting includes cell lysis, protein quantification, SDS-PAGE, protein transfer, antibody incubation, and detection. Enables detection and quantification of target proteins.
Data Acquisition and Analysis of Derivatized Hexose/Glucose by LC-QQQ-MS
By onProtocol for analyzing derivatized hexose/glucose with LC-QQQ-MS using a C18 column and Ultivo mass spectrometer. Includes chromatographic conditions, MS settings, and data analysis for high-throughput quantification.
