Expression and purification of ATP13A4 from Saccharomyces cerevisiae
By onThis protocol describes the expression and purification of human ATP13A4 with a C-terminal Twin-Strep tag from Saccharomyces cerevisiae using a CuSO₄-inducible system.
Purification of ATP13A4 from HEK293T cells
By onThis protocol describes the purification of ATP13A4 from HEK293T cells stably overexpressing ATP13A4. The procedure includes cell lysis, membrane solubilization, and affinity purification using Strep-TactinXT resin.
Lentiviral vector production and primary astrocyte transduction
By onProtocol for lentivirus production & transduction into astrocytes is outlined, allowing efficient transduction & stable selection. Facilitates research on primary gene function in astrocytes.
