Patch-clamp recordings of SNpc dopamine neurons from midbrain slices
By onProtocol for patch-clamp recordings of SNpc dopamine neurons from midbrain slices
Immunostaining and Western blot images
By onRaw immunostaining and western blot images included in Adami et al, 2025.
Dynamic basal ganglia output signals license and suppress forelimb movements-Datasets
By onThis repository contains the datasets and analysis files associated with the study by Falasconi, Kanodia, and Arber (2025).
all data related to “Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven by PRKN knockout”
By onRaw imaging data for "Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven by PRKN knockout"
Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
By onThis protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices.
Imaging axonal calcium dynamics in ex vivo mouse brain slices
By onProtocol for imaging calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices using GCaMP6f in DAT-Cre:Ai95D mice. Calcium transients were observed in response to single and train electrical stimuli.
Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
By onProtocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
iPSC QC (Adami et al, 2025)
By onSummary table and data from iPSC quality control experiments in Adami et al. 2025 are accessible for review.
Three antibody staining protocol for immunolabeling of mouse brain sections
By onThe protocol addresses factors affecting imaging of tissue sections: autofluorescence and consistency in antibody preparation for ideal results.
Synaptic analysis workflow using SEQUIN
By onProtocol for analyzing synaptic loci in neuroscience experiments using Zeiss Airyscan microscope. Includes identification, quantification, and localization of synaptic loci and nearby labels on neurites of the super-resolution images acquired
PET-CT related to “Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits”
By onPET-CT related to "Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits"
Generation of isogenic iPSC lines through CRISPR/Cas9-Mediated Targeting
By onProtocol for creating genetically modified human pluripotent stem cell lines using CRISPR/Cas9: Guide RNAs inserted into pX330 Cas9 plasmid, delivered with targeting vectors into hPSCs.
Primary data2: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By on*LRRK2* mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as found in the manuscript.
Mouse brain immunohistochemistry – colorimetric analysis
By onProtocol for colorimetric analysis of mouse brain immunohistochemistry is described.
Primary data1: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By onPathogenic LRRK2 mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as indicated in the primary data for the manuscript.
Part2: PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease
By onPublication seeks extra microscopy images for ChAT+ ciliation experiments, focusing on PPM1M, a phosphatase potentially linked to Parkinson's disease via LRRK2 and phosphoRab12.
Confocal microscopy of DFP-treated cells
By onThis protocol details the confocal microscopy of DFP-Treated cells after fixation and staining.
Zenodo_In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment
By onZenodo deposition for In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment_EMPIAR-12871
By onDataset of Oligomycin / Antimycin A treated mitochondria from U2OS cells
Microwave assisted sample processing with cultured cells for 3D FIB-SEM
By onRoutine procedure for microwave assisted sample processing of biological tisses for 3D FIB-SEM.
