Live-cell imaging for synaptic vesicle precursors in human iNeuron axons
By onThe authors describe procedure and equipment used for live-imaging of synaptic vesicle precursors.
Live-cell imaging
By onLive-cell imaging is a technique to visualize dynamic cellular processes in living biological samples.
Image processing of full-length monomeric LRRK2
By onThis protocol assumes that 2000 to 4000 movies of full-length LRRK2 embedded on vitreous ice are collected with an electron microscope equipped with a direct detector. It focuses primarily on the monomeric population of LRRK2 and has been leading to 3-4 Å resolution structures.
Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios
By onThis quick guide provides key minimal steps for preparing the Titan/SerialEM for the tomogram data collection on lamella or in vitro specimens with a K3 camera. paceTOMO routine is also included for a typical tomogram data collection session. Please note that this is not an exhaustive guide, but summarises the order of key steps.
Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation
By onHere, the authors examine the structural interaction between ATG9A and components of the ULK1 complex to better understand the process of the PINK1- and Parkin- dependent mitophagy pathway implicated in Parkinson's disease.
Microscopy-based evaluation of Parkin translocation and mitophagy in FBXO7-/- cell lines
By onAssociated with preprint:
Microscopy-based mitochondrial morphology measurements in iNeurons
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Immunostaining of iPSC-derived neurons
By onThe authors fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal and axonal abundance of proteins of interest.
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
By onNeuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Stereology-mediated cell counting using StereoInvestigator
By onProtocol for stereologic count with StereoInvestigator.
ALS and FTD-associated missense mutations in TBK1 differentially disrupt mitophagy
By onSource data for manuscript https://doi.org/10.5281/zenodo.4670341
3D FIBSEM CLEM
By onThis protocol details the procedure of Correlative Light Microscopy and Electron Microscopy (CLEM) with 3D Focus Ion Beam Scanning Electron Microscopy (FIBSEM) technique in Zeiss Crossbeam 550 FIBSEM system.
Live-cell imaging; mitochondrial reactive oxygen species
By onThis protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye.
Intracellular neuromelanin quantification
By onProtocol for intracellular neuromelanin quantification.
Preparation of LRRK1 RCKW cryo-EM grids
By onProtocol used to create LRRK1 RCKW grids for cryo-EM used in Snead, Matyszewski, Dickey et al.
Light microscopy immunoperoxidase staining
By onProtocol for Light microscopy immunoperoxidase staining.
Live imaging to investigate mitophagy kinetics and NEMO recruitment in HeLa-M cells
By onProtocol for live imaging to investigate mitophagy kinetics and NEMO recruitment in HeLa-M cells.
