Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
By onA new method allows monitoring dopamine release in mice with high spatial and temporal resolution in multiple locations in the striatum simultaneously using a fluorescent sensor. The protocol involves array fabrication and pre-implant mapping.
DAB ANTIBODY (IHC) STAINING PROTOCOL
By onThis is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
Rat brain processing for histological analyses
By onProtocol for rat brain processing for histological analyses.
Brain Slice Preparation for electrophysiology recording
By onThis protocol describes brain slice preparation for electrophysiology recording.
Sample preparation for aCGH karyotyping
By onThis protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
Immunohistochemistry using paraffin embedded tissue
By onImmunohistochemistry using paraffin embedded tissue
Stereotaxic rat brain surgery for Substantia Nigra targeting
By onProtocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Endosome isolation
By onSubcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Nuclei Isolation and Sorting from Frozen Human Temporal Cortex V2
By onProtocol focuses on isolating and sorting nuclei from frozen human temporal cortex.
Perfusion-fixation procedure for adult rhesus monkeys
By onThe protocol details the perfusion-fixation procedure for rhesus monkeys
Standard Operating Procedure: Mouse Stereotaxic Intracranial Injection Surgery
By onSOP outlines procedures for mouse stereotaxic intracranial injection surgery.
Purification and Crystallization of ATG9 HDIR-ATG101:ATG13 complex
By onPurification and Crystallization of ATG9 HDIR (828-839) fused ATG101 (1-198):ATG13 (1-197).
Preparing of genomic DNA from in vitro cultured cells
By onThis protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
By onThis protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Post mortem human substantial nigra TH staining
By onThis protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
Crystallization, data collection and structure solution of PxP-VAB
By onProtocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
