Stereotaxic Surgery for Viral Injections or Brain Implant
By onThis protocol details stereotaxic surgery for injection of AAVs or implantation of fiber photometry probe(s) in mouse brain.
Transcardial Perfusion for Immunohistochemistry
By onThis protocol details transcardial perfusion of mice with PBS followed by 4% Paraformaldehyde
Collection of mouse cerebrospinal fluid from the cisterna magna
By onThis protocol describes the collection of mouse cerebrospinal fluid from the cisterna magna.
Preparation of total cell lysates
By onThis protocol outlines a step-by-step method for preparing cell lysates.
RNA isolation and cDNA synthesis
By onThis protocol details the isolation of RNA, purification, and subsequent cDNA synthesis for downstream applications such as qPCR.
Nuclei isolation from brain tissue for single-cell ATAC
By onProtocol for isolating nuclei from brain tissue for single-cell ATAC assays like 10X Genomics Chromium Next GEM Single Cell ATAC Kit, HyDrop-ATAC, etc.
Nuclei isolation of fresh frozen human brain samples for for scRNA-seq
By onProtocol for isolating nuclei for single-cell RNA sequencing is detailed.
HyDrop-v2-ATAC
By onProtocol covers nuclei preparation, ATAC tagmentation, microfluidics setup, HyDrop run, PCR1 cleanup, library purification, and quality control for efficient processing.
DAB ANTIBODY (IHC) STAINING PROTOCOL
By onThis is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
Rat brain processing for histological analyses
By onProtocol for rat brain processing for histological analyses.
Brain Slice Preparation for electrophysiology recording
By onThis protocol describes brain slice preparation for electrophysiology recording.
Sample preparation for aCGH karyotyping
By onThis protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
Immunohistochemistry using paraffin embedded tissue
By onImmunohistochemistry using paraffin embedded tissue
Stereotaxic rat brain surgery for Substantia Nigra targeting
By onProtocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Endosome isolation
By onSubcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Nuclei Isolation and Sorting from Frozen Human Temporal Cortex V2
By onProtocol focuses on isolating and sorting nuclei from frozen human temporal cortex.
