Immunohistochemistry & Microscopy-Falasconi & Kanodia et al 2025
By onImmunohistochemistry and microscopy methods used in Falasconi & Kanodia et al 2025.
AAV Production Protocol-Arber Lab
By onAAV Production methods used in Falasconi & Kanodia et al 2025 and is the general protocol for Arber Lab.
Preparation of tissue punches of SNpc for the analyses of mitochondrial function
By onProtocol for the preparation of tissue punches of SNpc for the analyses of mitochondrial function
Preparation of midbrain slices for electrophysiology
By onProtocol for the preparation of midbrain slices for electrophysiology
Stereotaxic Surgery
By onThis protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
RNA extraction from organoids
By onProtocol used in the Gradinaru lab for RNA extraction from organoids
Copy of RNA extraction from mouse tissue
By onProtocol used in the Gradinaru lab for RNA extraction from mouse tissue
Immunocytochemistry (Henderson Lab)
By onProtocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Histology Fushiki et al 2024
By onHistology protocol for Fushiki et al 2024 , to quantify Th+ cells and visualize the dorsal-tier or ventral-tier of SNc DANs.
Immunohistochemistry (Histology + Imaging)-Mendelsohn et al 2025
By onThe protocol outlines the steps for preparing mouse brain tissue for immunostaining and imaging from Mendelsohn et al 2025.
In Situ Hybridization -Mendelsohn et al 2025
By onThis protocol outlines the steps for preparing mouse brain tissue for In Situ Hybridization from Mendelsohn et al 2025.
RNA In situ hybridization (ISH) using RNAscope®
By onThis protocol outlines the procedures for RNA in situ hybridization to simultaneously detect up to three targets using the RNAscope® Multiplex Fluorescent v2 assay (ACD).
DAB ANTIBODY (IHC) STAINING PROTOCOL
By onThis is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
Rat brain processing for histological analyses
By onProtocol for rat brain processing for histological analyses.
Brain Slice Preparation for electrophysiology recording
By onThis protocol describes brain slice preparation for electrophysiology recording.
Sample preparation for aCGH karyotyping
By onThis protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
