Section 1: Enzymatic DNA Fragmentation (Manually)
By onThis protocol details manual enzymatic DNA Fragmentation prior to Section 2: NGS library preparation for sequencing.
Section 2: NGS library preparation for sequencing
By onThis protocol details NGS library preparation for sequencing and should be performed after Section 1: Enzymatic DNA Fragmentation (Manually).
Targeted detection of SNCA CNVs in SOX10+ nuclei from oligodendrocytes containing alpha-synuclein inclusions isolated from human post-mortem brain
By onThis protocol describes the detection of low-level genomic mosaics in the human brain using DNA immunofluorescence-fluorescence in situ hybridisation, known as immuno-FISH, to analyze copy number variants in the SNCA gene related to PD & MSA.
Preparation of fresh paraformaldehyde for mouse perfusion and tissue fixation
By onPreparation of fresh paraformaldehyde for mouse perfusion and tissue fixation.
Making Carbon-Fibre Microelectrode (CFM) for electrochemical recordings of monoamines in ex vivo mouse brain slices
By onThis protocol is to make carbon-fiber microelectrode (CFM) for electrochemical recordings of monoamines in ex-vivo mouse brain slices.
Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
By onThis protocol describes a method to detect ubiquitination on a protein of interest. This technique relies on immunoprecipitation (IP) of ubiquitinated proteins from a cell lysate through the use of an anti-ubiquitin nanobody coupled to agarose beads. The eluate can be run on SDS-PAGE to determine whether the protein of interest was recovered in the IP and, therefore, ubiquitinated.
Immunofluorescence free-floating rat brain cryosections
By onProtocol for immunofluorescence on rat brain cryosections
Immunohistochemistry free-floating rat brain cryosections
By onProtocol for immunohistochemistry on rat brain cryosections.
Coupling of TMEM192 antibody to MyOne™ Epoxy Dynabeads™
By onThis protocol describes the coupling of rabbit monoclonal TMEM192 antibody to MyOne™ Epoxy Dynabeads™ . The coupled beads generated using this protocol can be used for the isolation of untagged lysosomes from cells and tissues.
Free floating immunofluorescence protocol on mouse brain sections for tau pathology
By onThis protocol describes the process of immunofluorescently labelling tau and phospho-tau in athymic mouse brains that have been grafted with human-disease derived iPSC's. This protocol can be adapted for other tissue types and antibody pairings.
Preparation and RNAscope-labeling of fresh mouse midbrain tissue
By onThe authors describe preparing and labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for probes targeting the genes Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By onPreparation of Holding ACSF Holding ACSF is used for incubating brain slices prior to cell-attached or whole-cell electrophysiological recordings of neurons.
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By onPreparation of Recording ACSF Recording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
Isolation of lysosomes using the Tagless LysoIP method in PBMCs
By onDetailed protocol for rapid isolation of intact lysosomes (including from clinical samples) for subsequent -omics analyses.
Sample preparation protocol for proteomic analysis of isolated lysosomes and whole cell extracts
By onHere, the authors describe a step-wise protocol for proteomic sample preparation derived from cell line models or isolated human cells. The protocol has been optimized for organelle pulldown preps.
Mouse tissue collection
By onThis protocol is about collecting mouse tissues for immunohistochemistry or biochemistry experiments. While it is mainly used for brain tissues, it also can be used for other visceral tissues.
Mouse sample collection for metabolomics studies
By onProtocol used in the Mazmanian lab for collecting brain and gut tissues and plasma from mouse for metabolomics.
