DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
By onThis protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
A method for RNA extraction, cDNA synthesis and and quantitative PCR from NIH-3T3 fibroblasts
By onProtocol for RNA extraction, cDNA synthesis, and qPCR from NIH-3T3 fibroblasts outlined. Method applicable to mouse embryonic fibroblasts as well.
Immunofluorescent Labeling of Preformed Fibril Protein Protocol
By onProtocol outlines use of AlexaFluor 488 kit to label alpha synuclein preformed fibrils for fluorescence, facilitating future applications.
Lysosomal isolation protocol
By onProtocol for isolating lysosomes from cultured cells is described.
Fecal Sample Collection
By onFecal sample collection from mice to use for gut microbiome sequencing analysis.
Protocol for non-human primate necropsy
By onA step-by-step guide explaining how to extract the brain and the spinal cord in non-human primates
Protocol for ventriculography-assisted stereotaxic surgery in non-human primates
By onProtocol for the intraparenchymal delivery of AAVs in the NHP brain by taking advantage of stereotaxic surgery assisted by intrasurgical ventriculography
Nissl Counterstaining – Cresyl Violet
By onNissl Counterstaining with Cresyl Violet for histological sections
Hematoxylin and Eosin (H&E) Staining
By onHematoxylin and Eosin (H&E) Staining for histological sections
Immunohistochemistry on free-floating cryosections
By onImmunochemistry protocol on rodent brain cryosections
Optical densitometry of neuronal fibers
By onOptical densitometry of striatal neuronal fibers in the rodent brain
Rodent brain processing for histological analyses
By onProtocol for rat brain processing in order to perform histological analyses
Brain Clarification for Neuromelanin visualisation by OPT
By onProtocol for the clarification of whole mouse brains to allow neuromelanin visualisation by Optical Projection Tomography
In situ Hybridization for Tyrosinase
By onIn situ hybridization protocol with DIG-labelled riboprobes
Human Brain Sequential Extraction (Tau)
By onHuman brain sequential detergent fractionation for PHF tau extraction
