Histology Fushiki et al 2024
By onHistology protocol for Fushiki et al 2024 , to quantify Th+ cells and visualize the dorsal-tier or ventral-tier of SNc DANs.
Immunocytochemistry (Henderson Lab)
By onProtocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Stereotaxic Surgery
By onThis protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
Copy of RNA extraction from mouse tissue
By onProtocol used in the Gradinaru lab for RNA extraction from mouse tissue
RNA extraction from organoids
By onProtocol used in the Gradinaru lab for RNA extraction from organoids
Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
Sample Preparation for Proteomic Analysis of Isolated Mitochondria and Whole-Cell Extracts
By onProtocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
AAV Production Protocol-Arber Lab
By onAAV Production methods used in Falasconi & Kanodia et al 2025 and is the general protocol for Arber Lab.
Preparation of midbrain slices for electrophysiology
By onProtocol for the preparation of midbrain slices for electrophysiology
Preparation of tissue punches of SNpc for the analyses of mitochondrial function
By onProtocol for the preparation of tissue punches of SNpc for the analyses of mitochondrial function
Immunohistochemistry & Microscopy-Falasconi & Kanodia et al 2025
By onImmunohistochemistry and microscopy methods used in Falasconi & Kanodia et al 2025.
Solutions used for FISH Codetection
By onProtocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
MS sample prep Quantitative whole cell proteomics
By onDescribes sample preparation procedure for Quantitative Whole Cell Proteome Analysis by Mass Spectrometry
Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
By onA new method allows monitoring dopamine release in mice with high spatial and temporal resolution in multiple locations in the striatum simultaneously using a fluorescent sensor. The protocol involves array fabrication and pre-implant mapping.
Perfusion and Histology Rodrigues-Vaz and Athalye et al, 2025
By onThis is the standard protocol for perfusion and histology of brain slices for Rodrigues-Vaz and Athalye et al, 2025.
Synaptic vesicle preparation from mouse brain
By onProtocol for isolating synaptic vesicles from mouse brains. Resulting SV-enriched fractions are used for biochemical analyses.
Mass spectrometry analysis with Data Independent Acquisition (DIA)
By onDetailed protocol for DIA-based MS data acquisition method is provided here.
