Synaptic vesicle preparation from mouse brain
By onProtocol for isolating synaptic vesicles from mouse brains. Resulting SV-enriched fractions are used for biochemical analyses.
Mass spectrometry analysis with Data Independent Acquisition (DIA)
By onDetailed protocol for DIA-based MS data acquisition method is provided here.
Rapid Isolation of Lysosomes (LysoIP) from mouse brain tissue
By onProtocol for rapid isolation of lysosomes from mouse brain tissue using LysoIP developed to assess lysosomal protein profiles. Enables detailed proteomic analysis of lysosomes, critical for biomolecule degradation and recycling.
Benzoyl-Hexose derivatization
By onHexose derivatization boosts hexose sugar detection in samples for LC-MS analysis.
BioID: Identifying Protein-Protein Interactions in Living Cells
By onBioID method uses BirA* to biotinylate proteins near a target protein in cells. This allows isolation of biotinylated proteins for identification using mass spectrometry.
Sample preparation for proteomic analysis of LysoIP
By onSample preparation in proteomics is vital for accurate data acquisition and analysis using techniques like LC-MS. Methods range from in-gel digestion to in-solution prep, all following similar principles and steps.
