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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Confirming circRNA expression by qPCR
This protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples.
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Protocol
Sample collection and processing for RNA analysis
This protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
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Protocol
Nuclei isolation and sorting from frozen human temporal cortex
This protocol is about nuclei isolation and sorting from frozen human temporal cortex.
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Protocol
Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons
This protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.
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Protocol
FM1-43 endocytic uptake assay in HIPSC derived neurons
When the cells undergo another stimulation (through KCL or electrical), the FM1-43 dye containing vesicles will be released through exocytosis, thus, resulting in a decrease in the FM1-43 fluorescence intensity.
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Themes