Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Datasets for Belal et al. ‘Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models’
Deposit contains processed data spreadsheets and SVG figure panels generated from the raw electrophysiology, imaging and in-situ hybridization recordings.
Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells
Assay for NBD-labeled lipid uptake in HeLa-CDC50A-OE cells includes seeding, transfection, and flow cytometry to measure flippase construct activity, enabling quantitative assessment of lipid uptake.
Flow cytometry data from the manuscript “Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells”
Summary: The manuscript presents a fluorescence-gated flow cytometry method to measure lipid flippase activity in mammalian cells.
Single-nucleus RNA (snRNA) sequencing
Step-by-step protocol describing the snRNA sequencing workflow for nuclei isolated from post-mortem human brain tissue.
Image resources (Garza et al. 2026)
Brightfield, Immunofluorescent images of KOLF2.1-derived microglia (MGlia) and RC17 hES cells derived dopaminergic neurons by Garza et al (2026).
iPSC QC (Garza et al, 2026)
Quality control reporting table and imaging files for hiPSCs and hESCs lines used in Garza et al., 2026.
Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency
Pathogenic variants in ATP13A2 cause Kufor-Rakeb syndrome & early-onset parkinsonism. Spermidine supplementation rescues symptoms by counteracting microglia dysfunction & improving neuronal integrity.
Dandiet: Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in Substanta nigra
This is a Dandiet that hosts binary files of electrophysiology data for the titled manuscipt 'Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in Substanta nigra'.
Mathematica code for Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in substantia nigra
Mathematica notebook containing functions used to measure spikerates, draw histograms, and extract current traces from Clampfit raw data exports.
Imaging Dataset for: A Vulnerable Subtype of Dopaminergic Neurons Drives Early Motor Deficits in Parkinson’s Disease
Dataset includes imaging data from experiments detailed in PMID: 39763754. Coronal brain sections imaged with fluorescence microscopy to assess reporter and transgene expression.
Fushiki et al – Video Recording of Parkinson’s Disease Model Mice
Raw behavioral datasets and experimental video recordings referenced in the paper( https://www.biorxiv.org/content/10.1101/2024.12.20.629776v1).
Histology from Rodrigues-Vaz and Athalye et al: Imaging of specific mouse brain region where viral injection and implant was placed at
This dataset is part of an experiment in which dorsolateral striatum (DLS) was imaged while mice performed task. This images show targeting of viral expression and lens implant to DLS.
EMG fabrication in Rodrigues-Vaz & Athalye et al
This is the protocol for fabrication of EMG electrodes for bulk muscle recordings used for Rodrigues-Vaz and Athalye et al, 2025 and based on Akay et al, 2006.
Virus Injections from Rodrigues-Vaz & Athalye et al
This is the standard stereotaxic/Nanoject injection mouse surgery protocol for Rodrigues-Vaz and Athalye et al, 2025.
Code used to analyze striatum population dynamics during isometric forelimb actions
Code used for analysis and figures for paper titled: "Striatal ensembles specify and control granular forelimb actions".
