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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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How Membrane Contact Sites Shape the Phagophore
Phagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.
FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Solutions used for FISH Codetection
Protocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
MANUAL CELL COUNTING
MANUAL CELL COUNTING
pCAG-FIP200 (1-640)-V314D-TSF
Mammalian expression of FIP200 NTD mutant
CAG-FIP200 (1-640)-R326D-TSF
Mammalian expression of FIP200 NTD mutant
pCAG-FIP200 (1-640)-R334D-TSF
Mammalian expression of FIP200 NTD mutant
pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF
Mammalian expression of FIP200 NTD mutant
pCAG-FIP200 (1-640)M3-TSF = V44D
Mammalian expression of FIP200 NTD mutant
pCAG-GST-ATG13 (460-517)-F466D/F470D
Mammalian expression of ATG13 (460-517) mutant
Copy of pCAG-GST-ATG13 (460-517)-F466D/F470D
Mammalian expression of ATG13 (460-517) mutant
pCAG-GST-ATG13 (460-517)-L476/L479D
Mammalian expression of ATG13 (460-517) mutant
pCAG-GST-ATG13 (460-517)-L495/L499D
Mammalian expression of ATG13 (460-517) mutant
pCAG-GST-ATG13 (460-517)-F509/F512D
Mammalian expression of ATG13 (460-517) mutant
pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D
Mammalian expression of ATG13 (363-398) mutant