Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Solid phase binding assay – Clusterin binding to very low-density lipoprotein receptor (VLDLR)

This protocol details how to monitor Clusterin binding to the very low-density lipoprotein receptor (VLDLR) by enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al., 2014.

Protocol

Analysis of glycosphingolipids from human plasma

The original qualitative method was published in 2004 and measured the oligosaccharides selectively released from glycosphingolipids using a ceramide glycanase enzyme derived from the medicinal leech. This is an updated protocol with the focus on achieving sensitive and reproducible quantitation of glycosphingolipids from human plasma samples

Protocol

Open field

This protocol details about Open Field procedure. This protocol details about Open Field procedure.

Protocol

Intramuscular PFF injection

This protocol details the intramuscular PFF injection procedure. This protocol details the intramuscular PFF injection procedure.

Protocol

Pole test

This protocol details pole test.

Protocol

Rotarod protocol

This protocol details the rotarod test. 

Protocol

DAB antibody (IHC) staining protocol

This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue. This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.

Protocol

Mouse perfusion

This protocol details the mouse perfusion procedure.