This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Solid phase binding assay – Clusterin binding to very low-density lipoprotein receptor (VLDLR)
This protocol details how to monitor Clusterin binding to the very low-density lipoprotein receptor (VLDLR) by enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al., 2014.
Teams
Themes
Protocol
Analysis of glycosphingolipids from human plasma
The original qualitative method was published in 2004 and measured the oligosaccharides selectively released from glycosphingolipids using a ceramide glycanase enzyme derived from the medicinal leech. This is an updated protocol with the focus on achieving sensitive and reproducible quantitation of glycosphingolipids from human plasma samples
Teams
Themes
Teams
Themes
Protocol
Open field
This protocol details about Open Field procedure. This protocol details about Open Field procedure.
Teams
Themes
Protocol
Intramuscular PFF injection
This protocol details the intramuscular PFF injection procedure. This protocol details the intramuscular PFF injection procedure.
Teams
Themes
Teams
Themes
Teams
Themes
Protocol
DAB antibody (IHC) staining protocol
This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue. This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
Teams
Themes
Teams
Themes
Protocol
ImmPRESS polymer detection following BaseScope in situ hybridization
Protocol for performing ImmPRESS immunostaining with alkaline phosphatase labeling after BaseScope In Situ Hybridization mouse brain sections.
Teams
Themes
Protocol
Western blot in mouse brain tissue for detecting pRab proteins
Protocol for detecting pRab proteins in mouse brain tissue.
Teams
Themes
Protocol
Immunofluorescence staining in mouse brain tissue sections
This protocol details the immunofluorescence staining in mouse brain tissue sections.
Teams
Themes
Protocol
3,3-Diaminobenzidine Tetrahydrochloride (DAB) immunohistochemistry on mouse brain tissue sections
This protocol details the staining for free floating mouse brain sections.
Teams
Themes
Protocol
Maintenance & differentiation: Embryonic mouse hippocampal cells (CLU198)
This protocol outlines the maintenance, differentiation, and harvesting of the CLU198 embryonic mouse hippocampal cell line.
Teams
Themes