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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Protocol
Acute striatal or midbrain fiber photometry in head-fixed mice
While this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
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Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
This behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
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Catalepsy test (Bar test)
The catalepsy test (bar test) was developed to test motor coordination and motor impairments.
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Create mask for axonal quantification analysis with FIJI
This protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
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Endosome isolation
Subcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
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Genotyping mice from ear clips
This protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
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Immunofluorescence staining for postmortem mouse brain tissue
This is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
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Isolation and processing of embryonic and postnatal brains
This protocol describes:
-Harvesting embryos from females from embryonic day 10 [e10] to embryonic day 18 [e18]
-Brain isolation of embryonic (e10-e18) and postnatal brains (p0-p30)
-Processing and freezing of embryonic and postnatal brains.
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Protocol
Open Field Test to assess motor coordination in a mouse parkinsonian model
The Open Field task is a simple sensorimotor test used to determine general activity levels, gross locomotor activity, and exploration habits in rodent models of CNS disorders.
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Pole Test to assess motor coordination in parkinsonian mice
The pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
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Rotarod Test to assess motor coordination in a mouse parkinsonian model
This protocol evaluates motor coordination in the rotarod assay in parkinsonian mice.
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Sectioning of mouse brain by cryostat
This protocol describes how to use the cryostat to prepare and slice mouse brain sections for Immunohistochemistry.
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