Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Whole mount dissection and staining of enteric nervous system
This protocol details whole mount dissection and staining of the enteric nervous system.
Teams
Regional mouse brain analysis (Modified QUINT)
This series of protocols has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow:
Note that the original QUINT workflow was generated by Yates and colleagues, and all credit for the development of these programs goes to that team.
Teams
Embryonic/Postnatal mouse neuron culture protocol
This protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Teams
α-Synuclein protein preparation (Large scale)
Protocol for large-scale preparation of recombinant α-synuclein protein.
Teams
Direct ELISA
This protocol details how to set up and run a direct ELISA on your protein of interest.
Teams
Human brain sequential extraction (Tau)
This protocol details human brain sequential extraction for pathological tau.
This protocol is an adaptation of the work of several labs.
Teams
Hematoxylin and Eosin Stain – FOR PARAFFIN-EMBEDDED TISSUE SECTIONS
This protocol details Hematoxylin and Eosin Stain for paraffin-embedded tissue sections.
Teams
Protocols for stereotaxic injections into mouse brain and ex-vivo electrophysiology
The protocol outlines the injection of α-Synuclin PFF and monomer into the mouse brain, followed by the preparation of acute slices from mice for whole-cell patch clamp recordings.
Teams
