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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Teams
Protocol
Designing sgRNA oligos and inserting guides into the GEARBOCS vector
Protocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.
Teams
Protocol
Culturing 3T3 cells for validating GEARBOCS constructs
This protocol is for culturing Cas9 NIH/3T3 cells for the validation of GEARBOCS 2.0 and similar constructs for use in in vivo assays.
Teams
Protocol
AAV production and purification
This protocol is for the production and purification of Adeno-Associated Virus. The protocol contains the necessary steps to produce AAVs from HEK293T cell cultures.
Teams