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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Calf intestine alkaline phosphatase (CIAP) treatment
Alpha-synuclein phosphorylated at serine 129 (PSER129) occurs in two pools, non-aggregated (physiological) and aggregated (disease). This protocol allows for the selective dephosphorylation of non-aggregated PSER129 and enhances the specificity and sensitivity immunodetection of aggregated PSER129. Thus, this protocol can be used to differentiate physiological from aggregated PSER129.
Teams
Protocol
Multiplex labeling with tyramide fluorophores (Free-floating tissues) – Killinger Lab 2024
This protocol details the multiplex labeling of free-floating tissues using tyramide fluorophores in the Killinger Lab (2024).
Teams
Protocol
Rotarod
Rotarod protocol optimized for mice. This test is made to assess motor coordination and balance. The results should show lower latency-to-fall numbers for mice with motor impairments.
Teams
Protocol
Purification of proteins from PFA-fixed samples
This protocol details the purification of proteins from PFA-fixed samples and the extraction of proteins from formalin-fixed tissues. Also includes the biotin pulldown prior to LC-MS/MS. Samples generated for this protocol have been used for mass spectrometry, immunoblotting, and pulldowns.
Teams