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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Striatal dopamine measurement through HPLC
Protocol for striatal dopamine measurement in mouse brain
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Protocol
Post mortem human substantial nigra TH staining
This protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
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Protocol
Cell viability assessment
This is the protocol for assessing cell viability using a MTS cell proliferation assay kit.
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Protocol
Chemical degradation and determination of pheomelanin and eumelanin markers
This is a protocol involving chemical oxidation and reduction methods followed by high-performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.
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Protocol
Determination of NM concentration
This is the protocol for determining neuromelanin concentration and data.
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Protocol
Human neuroblastoma cell line SH-SY5Y culturing
This is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
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Protocol
Immunofluorescent staining for neuronal marker MAP2
This is the protocol for immunofluorescent staining for neuronal marker MAP2.
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Protocol
LC/MS analysis of plasma samples from PPMI
Plasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson’s disease and its pathophysiology.
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Protocol
Cytotoxicity Assay Protocol
This is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
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Protocol
Genetic modification of human induced pluripotent stem cells (hiPSCs) by lentiviral transduction protocol
This is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.
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Protocol
T cell differentiation from mice spleen tissue
This protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.
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Protocol
Unilateral intranigral AAV alpha synuclein mouse model
This protocol details methods for the unilateral intranigral AAV alpha-synuclein injection.
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