This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Differentiation of SH-SY5Y cells
This protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid.
Teams
Protocol
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells (iPSCs).
Teams
Protocol
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
Teams
Protocol
Mitochondria purification
This protocol describes how to purify mitochondria from cell culture.
Teams
Protocol
Membrane and cytosol fractionation
This protocol describes membrane and cytosol fractionation of cells expressing different DNAJC5 isoforms.
Teams
Protocol
In vitro depalmitoylation assay
This protocol describes the in vitro depalmitoylation assay of DNAJC5.
Teams
Protocol
CRISPR/Cas9 genome editing
This protocol describes the generation of DNAJC5 KO using CRISPR/Cas9 edition.
Teams
Protocol
Medium fractionation and EV preparation
This protocol describes the characterization of the extracellular alpha-synuclein and DNAJC5.
Teams
Protocol
Co-immunoprecipitation
This protocol describes a common procedure to perform co-immunoprecipitation.
Teams
Protocol
Making Carbon-Fibre Microelectrode (CFM) for electrochemical recordings of monoamines in ex vivo mouse brain slices
This protocol is to make carbon-fiber microelectrode (CFM) for electrochemical recordings of monoamines in ex-vivo mouse brain slices. This protocol is optimized for the detection of dopamine from 300 µm coronal striatal sections, using a Miller voltammeter.
Teams
Protocol
shRNA knockdown
This protocol describes a standard procedure to generate a stable cell line for a DNAJC5 knockdown using shRNA.
Teams
Protocol
Immunoblots
The steps of this protocol are similar for different sources of protein such as cell lysate, cytosol, or membrane samples, and EV samples. However, some specific changes depend on the protein of interest, endogenous alpha-synuclein, or type of cells, hiPSC dopamine neurons.
Teams
Protocol
Unconventional secretion of alpha-synucein mediated by palmitoylated DNAJC5 oligomers
This protocol, carried out according to the manufacturer’s instructions, uses a classical ELISA experimental design with sensitive electrochemical detection to provide a measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Teams
Protocol
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices
This protocol is to assess whether a drug changes the dopamine concentration released following a single pulse (1p) electrical stimulation.
Teams