Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Single cell transcriptomics from DAT-Cre mouse gut tissue
Colonic myenteric cells were dissociated, and live tdTomato-positive cells were FACS-collected. Colons were isolated from 2 male and female wild type adult DAT-Cre mice. Wild type C57Bl6/J was used as control for gating. Cells were processed according to 10X Genomics Chromium single cell 3′ Reagent guidelines.
Teams
Raw tabular data of confocal microscopic images for “Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content”
Using immunofluorescence, the authors uncovered a subpopulation of tdTomato-immunoreactive neurons in the ENS exhibit dual-transmitter content (DAT/ChAT, DAT,nNOS).
Teams
