Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
High-yield monocyte/macrophage differentiation from hiPSC
Here, the protocol describes a highly efficient method for differentiating monocytes/macrophages from hiPSC.
Teams
Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”
This is a collection of protocols for the manuscript “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.”
Teams
Protocols for “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice”
This is a collection of protocols associated to the manuscript “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice.”
Teams
Mitochondrial antigen presentation in RAW macrophages
This protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). Watch an interview about this protocol with Ahmed Fahmy, PhD.
Teams
Mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay
This protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo
Teams
Isolation of brain infiltrating lymphocytes
This protocol details the isolation of brain infiltrating lymphocytes after adoptive transfer of mitochondrial antigen- specific T cells. Splenic lymphocytes are screened to verify the presence/phenotype of CD8 in the periphery.
Teams
Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
This protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma.
Teams
T cell purification and activation
This protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen.
Teams
Perfusion/fixation for cryostat slicing for HHC
This protocol details the Perfusion and Fixation for cryostat slicing for HHC.
Teams
Immunohistochemistry of free floating slices
This protocol details the immunohistochemistry of free-floating slices.
Teams
DAB immunohistochemistry (IHC) staining for stereological analysis
This protocol details the DAB immunohistochemistry staining for stereological analysis of 40 µM slices cut with cryostat and stored in antifreeze.
Teams
Behavioral analyses in mice
This collection of protocols describes different behavioral assays performed in mice, including grip strength test, pole test, rotarod, open field, and nose poking learning task.
Teams
Brain infiltrating leukocytes (BILs) extraction
This protocol details the extraction of brain infiltrating leukocytes (BILs).
Teams
