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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Live imaging of primary mouse neuron cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
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Protocol
Immunostaining mouse brain tissue or neuronal cultures
This protocol describes immunohistochemical and immunocytochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
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Protocol
Ex Vivo Electrophysiology
This protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
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Protocol
Fiber Photometry (Mouse)
This protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
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Protocol
Immunohistochemistry
This protocol describes immunohistochemical staining of fixed brain sections.
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Protocol
In Vivo Electrophysiology (Mouse)
This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
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Protocol
Optogenetic Manipulation (Mouse)
This protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
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Protocol
Mouse Stereotaxic Surgery
This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
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Protocol
Fast Scan Cyclic Voltammetry (FSCV) in mouse brain slices
This protocol describes the method of Fast Scan Cyclic Voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.
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Protocol
Behavioral Testing – Open Field and Dyskinesia Scoring
This protocol describes behavioral testing to assess motor deficits in mice. It includes open field locomotor testing and scoring of levodopa-induced dyskinesia.
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Protocol
Mouse behavior – Open Field and T-Maze
This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
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Protocol
Mouse brain tissue collection and analysis
This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain.
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Protocol
Deep Brain Stimulation (DBS) Implant
This protocol describes the steps for making implants used for deep brain stimulation (DBS) in rodents.
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