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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Teams
Protocol
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
This protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo.
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Protocol
Immunohistochemistry of AAV-treated tissues
This protocol outlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.
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Protocol
Tissue handling for mice, marmoset, and rhesus macaques injected with AAV
This protocol outlines all procedures for handling of tissue of animal subjects treated with AAV.
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Protocol
Multiple sequence alignment
Procedure for multiple sequence alignment used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
ColabFold protein structure prediction and membrane interaction modeling
Procedure for ColabFold protein structure prediction and membrane interaction modeling used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Multi-electrode array (MEA)
Procedure for multi-electrode array used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Proteome analysis
Procedure for proteome analysis used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
Procedure for Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Proteomics: On-bead/in-solution digestion and phosphopeptide enrichment
Procedure for On-bead/in-solution digestion and phosphopeptide enrichment used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Preparation of an enriched synaptic vesicle fraction
Procedure for preparing enriched synaptic vesicle fractions used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Preparation and administration of systemic 4-AP
Procedure for preparation and systemic administration of 4-AP used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
Preparation of brain and Neuro2A lysates for biochemical assays and evaluation
Procedure for preparing and Neuro2A lysates for biochemical assays and evaluation used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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Protocol
CRISPR knock-in validation
Procedure for CRISPR knock-in validation used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function.”
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