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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Histology and retention of implanted carbon fiber thread electrodes in fixed tissue
Methods to cut and retain implanted carbon fiber electrodes in brain tissue.
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Protocol
Characterization of splay characteristics of carbon fiber electrode threads (CFETs) in agar brain phantom
Methods to measure deflection characteristics of carbon fiber electrodes in agar brain phantoms.
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Protocol
Maintenance of the recording chamber for non-human primate (Chamber cleaning and dura scraping)
Once a craniotomy has been done in the chamber, the inside of the chamber must be cleaned regularly (2-3 times per week) to prevent infection. This protocol explains two procedures to maintain the recording chamber in a healthy condition:(1) chamber cleaning and (2) dura scraping.
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Protocol
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 1 (Head fixation post and recording chamber implantation)
The performing surgical procedures under strictly sterile conditions is the key to the successful progress of the project.
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Protocol
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
Outlined are the steps to complete a craniotomy in an existing recording chamber placed on a cranial implant. This procedure can be done concurrently with the implant surgery (not recommended), or can take place upon recovery from the implant surgery.
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Protocol
Electrophysiological recording from a chronic chamber-implanted non-human primate
This protocol describes the preparation and insertion of extracellular recording electrodes into intracranial targets of a non-human primate implanted with a chronic recording chamber. A piercing guide cannula is used to penetrate the dura and to guide the electrode. Practices related to targeting, electrode cleaning, and electrode movement and settling relative to brain tissue are discussed.
Teams
Protocol
Standard operating procedure (SOP) for combined ICA and systemic administration of MPTP: The overlesioned (Bilateral Asymmetric) non-human primate model
This SOP describes the procedures for the combined internal carotid artery (ICA) and systemic (intravenous or intramuscular) administration of MPTP in non-human primates. This model produces an overlesioned or bilateral asymmetric model, induced by unilateral ICA plus systemic administration.
This protocol has been adapted from Bankiewicz et al. (1999).
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Protocol
Immunohistochemistry for carbon fiber thread electrodes
Methods for immunofluorescent staining of brain tissue with indwelling electrodes.
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Protocol
In vitro FSCV testing of carbon fiber electrodes to characterize functional operation in dopamine detection
Methods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.
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Protocol
In vivo carbon fiber electrode thread (CFET) implantation and testing procedures
Procedures for implanting and testing CFET devices in rats are described.
Teams
Protocol
Standard DAB staining for free-floating fixed NHP brain tissue
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
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Protocol
DAB staining for GFP on free-floating fixed NHP brain tissue
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect green fluorescent protein (GFP).
Teams
Protocol
In-vivo MRI and CT scanning protocol for non-human primates using a 3D-printed MR-compatible stereotaxic frame
This protocol describes the methods of using a custom 3D printed stereotaxic frame to acquire MRI and CT scans for nonhuman primates in detailed steps.
Teams